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Cell-Based Assay

Real-time cell imaging for the target of lead substance as new drug in signal transduction mechanism

01 Real-time cell/FRET imaging

Real-time cell imaging for the target of lead substance as new drug in signal transduction mechanism

Fluorescence Resonance
Energe Transfer(FRET)

Example

Calcium imaging
PKA activation
AMPK activation
ATP imaging
PI(3,4,5)P3 imaging

02 Confocal imaging

Confocal laser scanning microscopy (CLSM) produces a high-resolution on the optical sections of each cell or tissue is achieved. Unlike conventional fluorescent microscope, CLSM provides a clearer fluorescent image, 3D image, time-lapse and kinetics image by using a light source (laser) and pinhole diameter effect.

Confocal Laser
Scanning Microscope

03 FCS/FCCS

– Quantify fluorescent variation signal that occurs in the confocal observation region
– Measure coupling intensity between drugs and reactants
– Measure absolute concentration of intra-cellular fluorescent substance, molecular size and shape and indirect molecular interaction

04 TEM/SEM/Immuno-EM service

These microscopes are ideal for life sciences and are able to yield information on cellular microstructure, location of targeted material in celluar microstructure, and microstructure of materials.

TEM (mitochondria / disease mouse)

TEM (drug carrier-cell reaction)

SEM (biliary stent & kidney)

Immuno-EM (exosome)

05 Correlative Light & Electron Microscopy

Correlative light electron microscopy (CLEM) combines the capabilities of two typically separate microcopy platforms: confocal microscopy and electron microscopy (EM).

Correlative Light &
Electron Microscopy : CLEM

06 High Throughput Screen