CELL PHONOTYPING IN TUMOR MICROENVIRONMENTS
Multiplex IHC Images
Pathology view Images
The Multiplex IHC assay is a new technique that allows simultaneous staining of multiple targets on one slide to study the correlation between anticancer agents and target molecules. General tissue staining is difficult to stain more than three targets on a slide due to the limitation of antibody use, but multiplex IHC analysis allows simultaneous staining of up to nine targets. In general fluorescence microscopy or confocal microscopy, it is difficult to use fluorescent dyes of near spectrum at the same time due to bleedthrough (wavelength overlap with excitation cross-talk and emission cross-talk). The multiplex IHC analysis method can be imaged with equipment capable of spectrum unmixing using an automated algorithm to obtain accurate results by separating autofluorescence of tissue as well as each intrinsic fluorescence. Multiplex IHC analysis captures information through imaging and automatically segments tissues and cells, automatically analyzing the association between each target and immune cells. In addition, this technology improves the general IHC quantification method, so that large-scale analysis and quantitative analysis of tissue samples can be accelerated, which can greatly help understanding the pathology of biomarkers in tissues, thereby greatly aiding the study of existing pathologies. The multiplex IHC assay is expected to play a pivotal role in the discovery of key targets and immune monitoring in the development of new drugs by analyzing the interrelationship between target marker-anticancer or immunotherapeutic-immune cells.